Part:BBa_K3971004

sps - Sucrose-phosphate synthase gene in Cyanobacteria.

Usage and Biology

The sps gene is native to the Synechococcus sp, and the sps catalyzed reaction is the rate limiting step in sucrose biosynthesis in cyanobacteria [1]. It catalyzes the rate-limiting conversion of uridine diphosphate glucose (UDP-Glu) and fructose 6-phosphate (F6P) into sucrose 6-phosphate (S6P) in the sucrose synthesis pathway. In Synechococcus elongatus UTEX 2973, the sps enzyme has both sucrose-phosphate synthase and sucrose-phosphate phosphatase activities [2].

Metabolic pathway of sucrose biosynthesis in Synechococcus elongatus UTEX 2973. Figure adapted from: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

References

1: Du, Wei, et al. "Exploring the photosynthetic production capacity of sucrose by cyanobacteria." Metabolic engineering 19 (2013): 17-25.

2: Lin, Po-Cheng, Fuzhong Zhang, and Himadri B. Pakrasi. "Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973." Scientific reports 10.1 (2020): 1-8.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 1676
  Illegal PstI site found at 1714
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1676
  Illegal NheI site found at 1869
  Illegal PstI site found at 1714
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1676
  Illegal XhoI site found at 1453
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 1676
  Illegal PstI site found at 1714
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 1676
  Illegal PstI site found at 1714
  
• 1000
  COMPATIBLE WITH RFC[1000]